Isolation, Characterization, and Formulation of Indigenous Beauveria bassiana Fungus Against Colorado Potato Beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

YILDIRIM K., ESKİ A., Biryol S., Erdoğan P., DEMİR İ.

Potato Research, cilt.67, sa.2, ss.583-601, 2024 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 67 Sayı: 2
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1007/s11540-023-09654-4
  • Dergi Adı: Potato Research
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.583-601
  • Anahtar Kelimeler: Biological control, Colorado potato beetle, Entomopathogenic fungi, Mycoinsecticide, Oil-based formulation
  • Bilecik Şeyh Edebali Üniversitesi Adresli: Evet

Özet

In this study, a total of 12 different fungal isolates were obtained from two different Colorado potato beetle (CPB) populations to identify a new effective agent to control L. decemlineata, which has developed resistance to many chemicals. The isolates were defined as Beauveria bassiana based on the ITS1-5.8S-ITS2, EF1-α, RPB1, and Bloc gene sequences. Under laboratory conditions, all isolates were pathogenic, but their virulence was different on larvae and adults. LdA-1 was the most virulent isolate with a mortality of 80% in larvae and 50% in adults. The LC50 value of this isolate was determined to be 0.2×106 and 0.17×108 conidia/ml for larvae and adults, respectively. Based on these results, LdA-1 isolates were used for mycoinsecticide development. Conidia were produced by solid-state fermentation using rice as a substrate. The conidia were formulated as oil-in-water emulsions and their efficacy was evaluated. The efficacy of the oil-based formulation against CPB larvae and adults was tested on eggplant in pot experiments. The new oil-based product caused 100% and 97% mortality on larvae and adults, respectively, at 1×108 conidia/ml. The LC50 value of our formulation for larvae and adults was calculated to be 1.2×106 and 0.2×107 conidia/ml, respectively. These results highlight that this formulation could be a suitable product for CPB control instead of conventional synthetic insecticides. Graphical Abstract: [Figure not available: see fulltext.]