UV-B induces cell death in the lichen Physcia semipinnata (J.F.Gmel) Physcia semipinnata (J.F.Gmel)' de UV-B radyasyonunun hücre ölümü üzerine etkisi

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ÜNAL D., UYANIKGİL Y.

Turkish Journal of Biology, cilt.35, sa.2, ss.137-144, 2011 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 35 Sayı: 2
  • Basım Tarihi: 2011
  • Doi Numarası: 10.3906/biy-0901-5
  • Dergi Adı: Turkish Journal of Biology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.137-144
  • Anahtar Kelimeler: Acridine orange staining, Lichen, TUNEL reaction, UV-B radiation
  • Bilecik Şeyh Edebali Üniversitesi Adresli: Evet

Özet

We have examined the consequences of Ultraviolet-B (UV-B) irradiation in the thallus of the lichen Physcia semipinnata (J.F.Gmel) in terms of cell viability and apoptotic-like formation. UV-B induced oligonucleosomal DNA fragmentation was detected by TUNEL assay and this is the first study showing DNA fragmentation in thalli. The intensity of TUNEL-positive cells after exposure to UV-B at doses up to 95.9 J/cm2 was higher in the photobiont layer than the mycobiont layer. © TÜBİTAK.