Akademik Veri Yönetim Sistemi
International Journal of Biological Macromolecules, cilt.316, 2025 (SCI-Expanded)
The main objective of the present study is to develop a novel and sustainable electrochemical biosensor for the monitoring of genotoxic effect of ziram. For this purpose, pencil graphite electrodes (PGEs) were modified with biopolyol obtained by liquefying olive pomace in polyhydric alcohols with an acid catalyst (BPL) and chitosan (CHIT), and the biointeraction between commercial form of ziram and DNA was performed at the surface of the CHIT/BPL-PGEs. Changes at the cathodic peak current (Ic) measured in 2.00 mM Fe(CN)63−/4- in 0.10 M KCl obtained by cyclic voltammetry (CV) were evaluated for BPL and CHIT modification. Electrochemical and microscopic characterization studies of the CHIT/BPL-PGEs were performed by CV, electrochemical impedance spectroscopy (EIS) and sscanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM-EDX). CHIT was effectively modified on BPL-PGE compared to unmodified PGE, and reproducible, repeatable and stable electrochemical response was obtained using CHIT/BPL-PGEs. Linear sweep voltammetry (LSV) was applied for nucleic acid detection, and the changes at the Ic measured by LSV were evaluated for genotoxicity assessment. Genotoxic effect of ziram could be monitored using CHIT/BPL-PGEs for <30 s with reliable results. The greenness level of the biosensor was found to be 0.79 using Analytical GREEnness Metric Approach and Software (AGREE) which indicated high sustainability.