An investigation of the genotoxic and cytotoxic effects of myclobutanil fungicide on plants

Creative Commons License

POYRAZ İ.

Plant Protection Science, cilt.58, sa.1, ss.57-64, 2022 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 58 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.17221/6/2021-pps
  • Dergi Adı: Plant Protection Science
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.57-64
  • Anahtar Kelimeler: Allium cepa, DNA-markers, Pesticides, Toxicity
  • Bilecik Şeyh Edebali Üniversitesi Adresli: Evet

Özet

© 2022, Czech Academy of Agricultural Sciences. All rights reserved.Myclobutanil is a chemical pesticide commonly used in the production of some vegetables and fruits like greenhouse peppers, grapes, and apples. The aim of this study was to investigate the genotoxic and cytotoxic effects of myclobutanil fungicide on the Allium cepa plant, the model organism. Randomly amplified polymorphic DNA and inter simple sequence repeat-PCR techniques were performed on the DNA of A. cepa exposed to the different myclobutanil doses and time periods. The nucleus anomalies and abnormal anaphases were investigated using a light microscope. PCR analyses showed that myclobutanil causes some DNA sequence changes on the onion genome depending on the increase in the fungicide dose and exposure time. It was determined that myclobutanil has a serious genotoxic effect, even in low doses like 25–50 ppm.