An investigation of the genotoxic and cytotoxic effects of myclobutanil fungicide on plants

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POYRAZ İ.

Plant Protection Science, vol.58, no.1, pp.57-64, 2022 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 58 Issue: 1
  • Publication Date: 2022
  • Doi Number: 10.17221/6/2021-pps
  • Journal Name: Plant Protection Science
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Page Numbers: pp.57-64
  • Keywords: Allium cepa, DNA-markers, Pesticides, Toxicity
  • Bilecik Şeyh Edebali University Affiliated: Yes

Abstract

© 2022, Czech Academy of Agricultural Sciences. All rights reserved.Myclobutanil is a chemical pesticide commonly used in the production of some vegetables and fruits like greenhouse peppers, grapes, and apples. The aim of this study was to investigate the genotoxic and cytotoxic effects of myclobutanil fungicide on the Allium cepa plant, the model organism. Randomly amplified polymorphic DNA and inter simple sequence repeat-PCR techniques were performed on the DNA of A. cepa exposed to the different myclobutanil doses and time periods. The nucleus anomalies and abnormal anaphases were investigated using a light microscope. PCR analyses showed that myclobutanil causes some DNA sequence changes on the onion genome depending on the increase in the fungicide dose and exposure time. It was determined that myclobutanil has a serious genotoxic effect, even in low doses like 25–50 ppm.