Non-growth inhibitory doses of dimethyl sulfoxide alter gene expression and epigenetic pattern of bacteria

TUNÇER S., GURBANOV R.

Applied Microbiology and Biotechnology, cilt.107, sa.1, ss.299-312, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 107 Sayı: 1
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1007/s00253-022-12296-0
  • Dergi Adı: Applied Microbiology and Biotechnology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, ABI/INFORM, Applied Science & Technology Source, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Compendex, Computer & Applied Sciences, EMBASE, Environment Index, Food Science & Technology Abstracts, Geobase, MEDLINE, Pollution Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.299-312
  • Anahtar Kelimeler: Bacteria, DMSO, Escherichia coli, FTIR, Methylome, Transcriptome
  • Bilecik Şeyh Edebali Üniversitesi Adresli: Evet

Özet

© 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.Abstract: Although dimethyl sulfoxide (DMSO) is a widely used solvent in scientific research, drug screening settings, and biomedical applications, its solvent (vehicle) effects on biological processes are overlooked. Using Escherichia coli as a model, we aimed to investigate and evaluate the effects of low-dose DMSO-driven changes in bacterial cells in a comprehensive and multifaceted manner by combining Fourier transform infrared spectroscopy analyses, analytical cell-biology approaches, and high-throughput sequencing. Here, we show that the non-toxic (1.0 and 2.5%, v/v) DMSO doses reduce the cellular levels of reactive oxygen species, change the cellular nucleic acid content and DNA topology, affect the global 5-methylcytosine pattern of the genome, and modulate gene transcription. These results indicate that even at non-toxic concentrations, DMSO is not inert: it can alter validity by changing or masking the assessed activity of the analyte. Besides, this manuscript does not only highlight that the low, non-toxic solvent doses of DMSO impinge on biological processes, including genome structure and function, but also, the high-throughput sequence data obtained during the study offer a platform for future research to elucidate the mechanism of epigenetically regulated genes in bacteria. Key points: • A clear-cut differentiation between the low-dose DMSO-treated and -untreated bacteria by PCA and LDA. • Drastic alterations in the DNA topology and nucleic acids of DMSO-treated bacteria. • Changes in transcriptome and epigenetic signatures with the low-dose DMSO.