Akademik Veri Yönetim Sistemi
Materials Today Chemistry, cilt.50, 2025 (SCI-Expanded, Scopus)
This study explores the development and evaluation of nanofiber membranes made of polyacrylonitrile and thermoplastic polyurethane with titanium dioxide nanoparticles for bacterial cell wall filtration in microfluidic chips, highlighting the importance of understanding intracellular functions for developing targeted therapies and diagnostics. It demonstrates the use of electrospinning to fabricate nanofibers with PAN and TPU solutions prepared at 14 wt% in dimethylformamide (DMF) and TiO2 incorporated at 1 wt%. It has been found that TiO2 modification increased surface roughness and bacteria interaction sites in smooth, uniform fibers. FTIR spectroscopy confirmed the successful integration of TiO2 without altering the fundamental chemical structures of PAN and TPU. Thermogravimetric analysis (TGA) showed TiO2 modification improved thermal stability and filtration performance by shifting surface properties from hydrophobic to hydrophilic. Filtration efficiency was evaluated using Escherichia coli DH5α as a model organism, with thermal lysis achieved via chemical and heat treatment methods. Filtrations were conducted using a lab-on-chip microfluidic system, and the PAN/TiO2 and TPU/TiO2 nanofibers demonstrated enhanced bacterial filtration and genomic deoxyribonucleic acid (gDNA) capture compared to their unmodified types. TiO2 enhances membrane functionality by likely generating reactive oxygen species (ROS) and increasing surface charge, facilitating bacterial cell wall degradation and filtration efficiency.