UV-B induces cell death in the lichen Physcia semipinnata (J.F.Gmel) Physcia semipinnata (J.F.Gmel)' de UV-B radyasyonunun hücre ölümü üzerine etkisi

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ÜNAL D., UYANIKGİL Y.

Turkish Journal of Biology, vol.35, no.2, pp.137-144, 2011 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 35 Issue: 2
  • Publication Date: 2011
  • Doi Number: 10.3906/biy-0901-5
  • Journal Name: Turkish Journal of Biology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.137-144
  • Keywords: Acridine orange staining, Lichen, TUNEL reaction, UV-B radiation
  • Bilecik Şeyh Edebali University Affiliated: Yes

Abstract

We have examined the consequences of Ultraviolet-B (UV-B) irradiation in the thallus of the lichen Physcia semipinnata (J.F.Gmel) in terms of cell viability and apoptotic-like formation. UV-B induced oligonucleosomal DNA fragmentation was detected by TUNEL assay and this is the first study showing DNA fragmentation in thalli. The intensity of TUNEL-positive cells after exposure to UV-B at doses up to 95.9 J/cm2 was higher in the photobiont layer than the mycobiont layer. © TÜBİTAK.