Boutique hotels can be defined as establishments that offer unique designs and personalized services in the hospitality sector. Unlike traditional hotels, they aim to provide an individualized accommodation experience with a limited number of rooms. The interior designs of boutique hotels balance aesthetic, functional, and ergonomic design elements. In this context, the study aims to analyze the interior designs of boutique hotel rooms using the example of Denizli Anım Boutique Hotel and examine their effects on the user experience. A mixed-methods approach was employed in the study, utilizing a literature review, field surveys, and interviews. The spatial arrangements of different room types in Denizli Anım Boutique Hotel were thoroughly analyzed regarding design dimensions. The study focused on key design components, including color and material choices, furniture arrangements, lighting designs, and spatial organization. The findings indicate that ergonomic and aesthetic approaches, which enhance user satisfaction, have been adopted in the hotel’s interior arrangements. Specifically, color palettes and material preferences contribute to the psychological relaxation of guests, while furniture arrangements and lighting designs create a comfortable experience. It was also observed that the spatial organization harmonized with the hotel's overall architectural identity. In conclusion, it was determined that a balanced consideration of aesthetics and functionality in the interior designs of boutique hotel rooms increases user satisfaction. Accordingly, adopting user-centered design approaches in boutique hotel projects is considered to provide an important model for future projects.