Electrochemical monitoring of surface confined interaction between 6-Thioguanine and DNA by using single-use graphite electrode
Journal of Electroanalytical Chemistry, vol.733, pp.33-38, 2014 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 733
- Publication Date: 2014
- Doi Number: 10.1016/j.jelechem.2014.08.012
- Journal Name: Journal of Electroanalytical Chemistry
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.33-38
- Keywords: 6-Thioguanine, Differential pulse voltammetry, DNA biosensor, Drug-DNA interaction, Pencil graphite electrode
- Bilecik Şeyh Edebali University Affiliated: Yes
Abstract
In the present work, the electrochemical detection of interaction between 6-TG and calf thymus double stranded DNA (dsDNA) was explored by using single-use pencil graphite electrode (PGE) in combination with differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques. Firstly, the electrochemical behaviour of 6-TG was investigated voltammetrically by using PGE, and the two oxidation signals of 6-TG were measured at +0.25 V and +0.78 V. Experimental parameters, such as 6-TG concentration and interaction time were optimized based on the changes at the 6-TG signal observed at +0.25 V since the latter one overlapped with the oxidation signal of guanine measured at +0.95 V. The results based on the impedimetric analysis for interaction between 6-TG and dsDNA were consistent with the voltammetric results. The electrochemical monitoring of surface confined interaction between 6-TG and single stranded Oligo (A)25, or double stranded Oligo (A)25-Oligo (T)25 was explored based on the changes at the oxidation signals of 6-TG and adenine by using DPV. The single-use graphite sensor represented a sensitive, rapid and cost effective detection protocol for electrochemical monitoring of the interaction between 6-TG and DNA.