Formulation and in vitro evaluation of topical nanoemulsion and nanoemulsion-based gels containing daidzein


UĞUR KAPLAN A. B., ÇETİN M., Orgul D., TAGHIZADEHGHALEHJOUGHI A., HACIMÜFTÜOĞLU A., Hekimoglu S.

Journal of Drug Delivery Science and Technology, vol.52, pp.189-203, 2019 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 52
  • Publication Date: 2019
  • Doi Number: 10.1016/j.jddst.2019.04.027
  • Journal Name: Journal of Drug Delivery Science and Technology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.189-203
  • Keywords: Daidzein, Nanoemulsion, Nanoemulsion-based gel, Skin cancer, Stability
  • Bilecik Şeyh Edebali University Affiliated: No

Abstract

Daidzein (DZ) has antioxidant, anti-inflammatory activities and also inhibits the growth of the cancer cells. The aim of this study is to formulate nanoemulsions (NEs) and nanoemulsion-based gels (NEGs) containing DZ, in order to evaluate their cytotoxic potential for topical application against melanoma. The NE formulations were prepared by using a high-pressure homogenization technique. Protasan ™ UP G 213 (1% w/w) was added to the NE formulation to prepare NEG formulations. Droplet size values were 151.12 ± 2.70 nm and 149.80 ± 3.52 nm for Blank-NE (B-NE) and DZ-NE, 191.48 ± 5.26 nm and 200.25 ± 11.09 nm for B-NEG and DZ-NEG, respectively. Positive zeta potential values for the NEG formulations were obtained in the presence of Protasan ™ UP G 213. All formulations had appropriate pH values for skin and also complied with zero-order release kinetics. The DZ release rates obtained for DZ-NE and DZ-NEG were 2.701 ± 0.265 and 1.325 ± 0.117 μg/cm2/hour, respectively. During the stability study, NE and NEG formulations had droplet sizes in the nano-range. All formulations maintained the cytotoxic effect of DZ against the melanoma cell line. NE and NEG formulations with a controlled release profile and nanometer droplet size may be useful for the topical use of DZ and the treatment of skin cancer.