Detection of p53 Gene by Using Genomagnetic Assay Combined with Carbon Nanotube Modified Disposable Sensor Technology

ÇONGUR G., Plucnara M., Erdem A., Fojta M.

Electroanalysis, cilt.27, sa.7, ss.1579-1586, 2015 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 7
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1002/elan.201400731
  • Dergi Adı: Electroanalysis
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1579-1586
  • Anahtar Kelimeler: Carbon nanotubes, Differential pulse voltammetry, DNA hybridization, Magnetic particles, P53 Gene
  • Bilecik Şeyh Edebali Üniversitesi Adresli: Evet

Özet

Electrochemical monitoring of DNA hybridization related to p53 gene sequence was investigated using genomagnetic assay combined with single walled carbon nanotube (SWCNT) modified pencil graphite electrodes (PGEs). The hybridization was performed either at magnetic beads (MB) surface or in solution. The enhanced guanine signal was obtained using SWCNT-PGEs compared to one obtained by unmodified PGEs. The selectivity of genomagnetic assay was tested under optimum conditions. The DLs were calculated as 0.88μM and 0.11μM for hybridization performed at MB surface and solution, respectively. This selective, practical and cost effective genomagnetic assay combined with SWCNT-PGEs is reported herein for the first time.