In vitro effects of pesticide exposure on the activity of the paraoxonase-1 enzyme from sheep liver microsomes

Koncuk Cebeci B., Alim Z., BEYDEMİR Ş.

Turkish Journal of Chemistry, vol.38, no.3, pp.512-520, 2014 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 38 Issue: 3
  • Publication Date: 2014
  • Doi Number: 10.3906/kim-1308-20
  • Journal Name: Turkish Journal of Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.512-520
  • Keywords: Dichlorvos, Fenoxaprop-p-ethyl, Imidacloprid, Inhibition, Lambda cyhalothrin, Paraoxonase
  • Bilecik Şeyh Edebali University Affiliated: Yes

Abstract

The organophosphate target enzyme paraoxonase-1 (PON1) has been extensively studied in toxicology. Pesticides are organophosphate compounds that are commonly used in agriculture. They are also used as nerve gases, such as sarin and soman. Therefore, the eécts of these compounds on enzymatic activity are well known. In the present study, we investigated the in vitro eécts of fenoxaprop-p-ethyl, lambda cyhalothrin, imidacloprid, and dichlorvos pesticides on sheep liver microsomal PON1. The enzyme was puriéd approximately 141-fold with a specific activity of 1822.22 EU/mg proteins. The pesticides inhibited sheep liver PON1 in vitro, and the IC50 values for these compounds were 0.0103, 0.069, 0.157, and 0.2 μM, respectively. © TÜBİTAK.