A sensitive spectrophotometric ellipsometry based Aptasensor for the vascular endothelial growth factor detection

KARİPER İ. A., Üstündağ Z., ÇAĞLAYAN M. O.

Talanta, cilt.225, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 225
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.talanta.2020.121982
  • Dergi Adı: Talanta
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, L'Année philologique, Aerospace Database, Analytical Abstracts, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, EMBASE, Food Science & Technology Abstracts, Linguistic Bibliography, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Anahtar Kelimeler: Aptamers, Biosensors, Breast cancer, Spectroscopic ellipsometry, VEGF
  • Bilecik Şeyh Edebali Üniversitesi Adresli: Evet

Özet

© 2020 Elsevier B.V.A sensitive and selective, aptamer and spectroscopic ellipsometry based sensor is reported here for the early diagnosis of breast cancer, which is a common type of cancer following lung cancer. It was aimed to develop a single-step and label-free assay for the sensitive and selective detection of VEGF165. To this end, two different aptamers and spectroscopic ellipsometry were used. In the presented study, by determining the appropriate aptamer immobilization conditions, the spectroscopic ellipsometry technique was successfully applied for the detection of VEGF165 at the range of 1 pM–1000 pM in the buffer. Aptasensors have a detection limit of 5.81 pM and 4.29 pM, respectively.