Synthesis and Biological Evaluation of Some Pyrazoline Derivatives Bearing a Dithiocarbamate Moiety as New Cholinesterase Inhibitors

ALTINTOP M. D., ÖZDEMİR A., KAPLANCIKLI Z. A., Turan-Zitouni G., TEMEL H. E., Ciftci G. A.

ARCHIV DER PHARMAZIE, cilt.346, sa.3, ss.189-199, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 346 Sayı: 3
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1002/ardp.201200384
  • Dergi Adı: ARCHIV DER PHARMAZIE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Index Chemicus (IC)
  • Sayfa Sayıları: ss.189-199
  • Anahtar Kelimeler: Acetylcholinesterase, Anticholinesterase activity, Butyrylcholinesterase, Dithiocarbamate, Pyrazoline, ALZHEIMERS-DISEASE, ANTIBACTERIAL, CYTOTOXICITY
  • Bilecik Şeyh Edebali Üniversitesi Adresli: Hayır

Özet

In the present study, new pyrazoline derivatives were synthesized via the reaction of 1-(chloroacetyl)-3-(2-furyl)-5-aryl-2-pyrazolines with sodium salts of N,N-disubstituted dithiocarbamic acids. Each derivative was evaluated for its ability to inhibit acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) using a modification of Ellman's spectrophotometric method. The compounds were also investigated for their cytotoxic properties using the MTT assay. The most potent AChE inhibitor was found as compound 7 followed by compounds 27 and 17, when compared with eserine. Compounds effective on AChE carry the 2-dimethylaminoethyl moiety, which resembles the trimethylammonium group and the ethylene bridge of acetylcholine. Among all compounds, compound 7 bearing 2-dimethylaminoethyl and 3,4-methylenedioxyphenyl moieties was also found to be the most effective inhibitor of BuChE. The MTT assay indicated that the effective concentration of compound 7 was lower than its cytotoxic concentration.