CoronaVac (Sinovac) COVID-19 vaccine-induced molecular changes in healthy human serum by infrared spectroscopy coupled with chemometrics

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Dogan A., GURBANOV R., Severcan M., Severcan F.

Turkish Journal of Biology, cilt.45, sa.Special Issue 1, ss.549-558, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: Special Issue 1
  • Basım Tarihi: 2021
  • Doi Numarası: 10.3906/biy-2105-65
  • Dergi Adı: Turkish Journal of Biology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Veterinary Science Database, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.549-558
  • Anahtar Kelimeler: Attenuated total reflection-Fourier Transform Infrared (ATR-FTIR), CoronaVac-Sinovac, COVID-19, Fourier Transform Infrared (FTIR) spectroscopy, Multivariate analysis, Vaccine
  • Bilecik Şeyh Edebali Üniversitesi Adresli: Hayır

Özet

© TÜBİTAK.From the beginning of the COVID-19 coronavirus pandemic in December of 2019, the disease has infected millions of people worldwide and caused hundreds of thousands of deaths. Since then, several vaccines have been developed. One of those vaccines is inactivated CoronaVac-Sinovac COVID-19 vaccine. In this proof of concept study, we first aimed to determine CoronaVac-induced biomolecular changes in healthy human serum using infrared spectroscopy. Our second aim was to see whether the vaccinated group can be separated or not from the non-vaccinated group by applying chemometric techniques to spectral data. The results revealed that the vaccine administration induced significant changes in some functional groups belonging to lipids, proteins and nucleic acids. In addition, the non-vaccinated and vaccinated groups were successfully separated from each other by principal component analysis (PCA) and linear discriminant analysis (LDA). This proof-of-concept study will encourage future studies on CoronaVac as well as other vaccines and will lead to make a comparison between different vaccines to establish a better understanding of the vaccination outcomes on serum biomolecules.