Rutin ameliorates mercuric chloride-induced hepatotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis


ÇAĞLAYAN C., Kandemir F. M., Darendelioğlu E., YILDIRIM S., KÜÇÜKLER S., Dortbudak M. B.

Journal of Trace Elements in Medicine and Biology, vol.56, pp.60-68, 2019 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 56
  • Publication Date: 2019
  • Doi Number: 10.1016/j.jtemb.2019.07.011
  • Journal Name: Journal of Trace Elements in Medicine and Biology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.60-68
  • Keywords: Apoptosis, Epidermal growth factor receptor, Hepatotoxicity, Inflammation, Mercuric chloride, Rutin
  • Bilecik Şeyh Edebali University Affiliated: No

Abstract

Objective: Mercury is a global environmental pollutant and is responsible for several organ pathophysiology including oxidative stress-induced liver disorders. Therefore, the present study was conducted to evaluate the potential ameliorative effects of rutin on mercury chloride (HgCl2)-induced hepatotoxicity in adult male rats. Methods: HgCl2 was intraperitoneally injected at a dose of 1.23 mg/kg body weight for 7 days alone or in combination with the orally rutin (50 and 100 mg/kg body weight). Results: Rutin treatment significantly improved liver function tests [alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT)], and increased activities of antioxidant defense system [catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx)] and glutathione (GSH) content. The histological alterations and epidermal growth factor receptor (EGFR) expression in the HgCl2-induced liver tissues were decreased by administration of rutin. Furthermore, rutin reversed the changes in levels of apoptosis and inflammation related proteins involving p53, Bcl-2 associated X protein (Bax), B-cell lymphoma-2 (Bcl-2), cytochrome c, nuclear factor kappa B (NF-κB), tumor necrosis factor-α (TNF-α), B-cell lymphoma-3(Bcl-3) and interleukin-1β (IL-1β), and inhibited p38α mitogen-activated protein kinase (MAPK) and cysteine aspartate specific protease-3 (caspase-3) activations. Conclusion: The data of the present study suggest that rutin effectively suppress HgCl2‐induced hepatotoxicity by ameliorating oxidative stress, inflammation and apoptosis.