The role of oxidative stress genes and effect of pH on methylene blue sensitized photooxidation of Escherichia coli


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Idil Ö., Macit I., Kaygusuz Ö., DARCAN C.

Acta Biologica Hungarica, cilt.67, sa.1, ss.85-98, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 67 Sayı: 1
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1556/018.67.2016.1.7
  • Dergi Adı: Acta Biologica Hungarica
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.85-98
  • Anahtar Kelimeler: E. Coli, Kat E, Oxy S, Photooxidation, Sod B, Sox RS
  • Bilecik Şeyh Edebali Üniversitesi Adresli: Evet

Özet

In this study, the survival time of wild type E. coli W3110 and 11 mutants was analysed with a plate count method in methylene blue added or control groups under daylight fluoroscence illumination (4950 lux) at different pH values (5.0, 6.0, 7.0, and 8.0) in phosphate buffer. As a result, while the number of bacteria did not decrease under photooxidative stress at pH 5.0 and 6.0 during a 6-hour incubation, the wild type and all mutants decreased more than 2 log. at pH 8.0, and approximately one log. at pH 7.0. It was determined that a 2 log decrease in wild type E. coli takes 3.7 h according to t99 value at pH 8, these values were 2.39 h in the kat E mutant, 2.64 h in the sox R mutant, 2.67 h in the oxy R mutant, 2.71 h in the sod B mutant, 3 h in the btu E mutant, 3.38 h in the zwf mutant and 3.40 h in the sox S mutant, respectively (p < 0.05). The roles of these genes were proved with complement tests. Finally, it is found that the effectiveness of photooxidative stress is in direct relation with pH, and the kat E, sox R, oxy R, sod B, btu E, zwf, and sox S genes are important for the protection against this stress.