Aptamer-based ellipsometric sensor for ultrasensitive determination of aminoglycoside group antibiotics from dairy products

ÇAĞLAYAN M. O.

Journal of the Science of Food and Agriculture, cilt.100, sa.8, ss.3386-3393, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 100 Sayı: 8
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1002/jsfa.10372
  • Dergi Adı: Journal of the Science of Food and Agriculture
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Agricultural & Environmental Science Database, Analytical Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, EMBASE, Food Science & Technology Abstracts, INSPEC, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, DIALNET, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.3386-3393
  • Anahtar Kelimeler: aminoglycoside antibiotics, aptamer, biosensors, ellipsometry, surface plasmon resonance, ultrasensitive analysis
  • Bilecik Şeyh Edebali Üniversitesi Adresli: Evet

Özet

© 2020 Society of Chemical IndustryBACKGROUND: Residual antibiotics taken along with food consumed through the food chain are the main cause of the super-bacteria and may damage organs such as liver and kidney. Therefore, monitoring residual antibiotic levels of products in the food chain is both important and a requirement. Maximum residual limits for kanamycin and neomycin are 150 ng mL−1 and 500 ng mL−1 respectively, which are challenging for most sensor platforms. In this paper, a novel method is presented for the determination of antibiotics residues in animal-derived foods. RESULTS: Aptamer-based kanamycin and neomycin biosensors based on the spectroscopic ellipsometer and the surface plasmon resonance-enhanced total internal reflection ellipsometer methods as transducing element were developed. Detection limits of both sensor platforms were in the 0.1–1 nmol L−1 ranges, and the detection range was between the detection limit and 1000 nmol L−1. CONCLUSION: Both ellipsometry-based aptasensors can be used as an alternative to the existing enzyme-linked immunosorbent assay-based method in terms of assay time (10 min), detection limit (0.22 ng mL−1 for neomycin and 0.048 ng mL−1 for kanamycin), and detection range. © 2020 Society of Chemical Industry.