Aptamer-based ellipsometric sensor for ultrasensitive determination of aminoglycoside group antibiotics from dairy products

ÇAĞLAYAN M. O.

Journal of the Science of Food and Agriculture, vol.100, no.8, pp.3386-3393, 2020 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 100 Issue: 8
  • Publication Date: 2020
  • Doi Number: 10.1002/jsfa.10372
  • Journal Name: Journal of the Science of Food and Agriculture
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Agricultural & Environmental Science Database, Analytical Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, EMBASE, Food Science & Technology Abstracts, INSPEC, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, DIALNET, Civil Engineering Abstracts
  • Page Numbers: pp.3386-3393
  • Keywords: aminoglycoside antibiotics, aptamer, biosensors, ellipsometry, surface plasmon resonance, ultrasensitive analysis
  • Bilecik Şeyh Edebali University Affiliated: Yes

Abstract

© 2020 Society of Chemical IndustryBACKGROUND: Residual antibiotics taken along with food consumed through the food chain are the main cause of the super-bacteria and may damage organs such as liver and kidney. Therefore, monitoring residual antibiotic levels of products in the food chain is both important and a requirement. Maximum residual limits for kanamycin and neomycin are 150 ng mL−1 and 500 ng mL−1 respectively, which are challenging for most sensor platforms. In this paper, a novel method is presented for the determination of antibiotics residues in animal-derived foods. RESULTS: Aptamer-based kanamycin and neomycin biosensors based on the spectroscopic ellipsometer and the surface plasmon resonance-enhanced total internal reflection ellipsometer methods as transducing element were developed. Detection limits of both sensor platforms were in the 0.1–1 nmol L−1 ranges, and the detection range was between the detection limit and 1000 nmol L−1. CONCLUSION: Both ellipsometry-based aptasensors can be used as an alternative to the existing enzyme-linked immunosorbent assay-based method in terms of assay time (10 min), detection limit (0.22 ng mL−1 for neomycin and 0.048 ng mL−1 for kanamycin), and detection range. © 2020 Society of Chemical Industry.