An Overview of Biosensors for the Detection of Patulin Focusing on Aptamer-Based Strategies


KÜÇÜK N., ŞAHİN S., ÇAĞLAYAN M. O.

Critical Reviews in Analytical Chemistry, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Review
  • Publication Date: 2023
  • Doi Number: 10.1080/10408347.2023.2172677
  • Journal Name: Critical Reviews in Analytical Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, Environment Index, Geobase, Metadex, Civil Engineering Abstracts
  • Keywords: Aptasensor, food safety, immunosensor, imprinted polymers, mycotoxin, patulin
  • Bilecik Şeyh Edebali University Affiliated: Yes

Abstract

Patulin is a low molecular weight mycotoxin and poses a global problem, especially threatening food safety. It is also resistant to processing temperatures and is commonly found in fruits and vegetables. Studies have shown that it has toxic effects on animals and humans and the severity of patulin toxicity depends on the amount ingested. Therefore, the consumption of contaminated products, especially in infants and children, is important. The maximum daily intake of PAT that can be tolerated is found to be 0.4 µg/kg body weight to prevent chronic effects and the maximum residue limits in food samples were given as 50 ng/g (∼320 nM). Conventional methods for the detection of PAT have many disadvantages such as the use of expensive equipment, the need for trained personnel, and complicated sample preparation steps. To this extent, various numbers of research have been conducted on selective and sensitive detection of patulin using biosensor platforms in various media. This review presents an overview of the current literature dealing with the studies to develop patulin-specific aptamer-based biosensors and adapts various immobilization methods to increase the sensor response using different nanomaterials. Furthermore, a comparison of biosensors with conventional methods is presented using analytical performance parameters and their practicality for the detection of patulin.