Biotechnology and Applied Biochemistry, vol.68, no.6, pp.1113-1119, 2021 (SCI-Expanded)
© 2020 International Union of Biochemistry and Molecular Biology, Inc.A voltammetric biosensor for acetylthiocholine (ATCh) and paraoxon detection was successfully developed. To achieve this goal, polypyrrole (PPy) was synthesized onto the platinum (Pt) electrode surface in 0.30 M oxalic acid solution containing 25 mM pyrrole. PPy-coated Pt (Pt/PPy) electrode surface was covered with chitosan (Chi) (Pt/PPy/Chi). The acetylcholinesterase (AChE) enzyme was immobilized on the Pt/PPy/Chi electrode surface to build a voltammetric biosensor (Pt/PPy/Chi/AChE). The storage stability of the biosensor was determined to be 72% even after 60 days. The operational stability was determined to be 94% after 20 consecutive measurements. For the biosensor, the linear range was determined to be 30–50 µM for ATCh and 0.46–1.84 nM for paraoxon. The limit of detection (LOD) was determined to be 0.45 µM for ATCh and 0.17 nM for paraoxon.