Isolation of quality total rna from the aromatic plant Origanum onites

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Poyraz İ., Sözen E., Arslanyolu M.

Zeitschrift fur Naturforschung - Section C Journal of Biosciences, cilt.65, sa.3-4, ss.266-270, 2010 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 65 Sayı: 3-4
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1515/znc-2010-3-415
  • Dergi Adı: Zeitschrift fur Naturforschung - Section C Journal of Biosciences
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.266-270
  • Anahtar Kelimeler: Origanum onites, RNA isolation, Secondary metabolites
  • Bilecik Şeyh Edebali Üniversitesi Adresli: Evet

Özet

We successfully used the guanidine isothiocyanate method for isolation of total RNA from leaf, stem, and root tissues of the aromatic plant Origanum onites. The RNA was extracted with TRI Reagent® at room temperature and was recovered by isopropanol precipitation. The isolated RNA was capable of reverse transcription. The extraction method described here does not require ultracentrifugation, and it is fast, simple, and effective. The procedure can be completed within 3 hours and may be applicable to other aromatic medicinal plants containing high amounts of phenolic compounds. © 2010 Verlag der Zeitschrift fur Naturforschung, Tubingen.