Comparing the effectiveness of two culture media techniques in the diagnosis of prosthetic joint infection


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Gedikbaş M., Sobay U., Eren M. B., Aşçı M.

JOURNAL OF CONTEMPORARY MEDICINE, cilt.15, sa.1, ss.30-35, 2025 (Hakemli Dergi)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 15 Sayı: 1
  • Basım Tarihi: 2025
  • Doi Numarası: 10.16899/jcm.1576595
  • Dergi Adı: JOURNAL OF CONTEMPORARY MEDICINE
  • Derginin Tarandığı İndeksler: Directory of Open Access Journals
  • Sayfa Sayıları: ss.30-35
  • Bilecik Şeyh Edebali Üniversitesi Adresli: Evet

Özet

Purpose: When infectious diseases are suspected, culture studies ensure the selection of the appropriate antimicrobial treatment and confirm the diagnosis. There are various differences in medium, sample collection technique, enrichment, and evaluation techniques.
Culture sampling makes important contributions to the diagnosis and treatment of prosthetic joint infections, the frequency of which has increased in recent years as a result of the increased rate of arthroplasty.
After evaluating the preoperative and intraoperative criteria together, prosthetic joint infections can be diagnosed in suspected cases without culture positivity.
This study aims to evaluate different culture samplings efficiency for the diagnosis of prosthetic joint infection. 
Methods: This study retrospectively evaluated 946 patients who had been sampled in our department between January 2005 and May 2015. These patients were divided into two groups according to their final diagnoses: group one (prosthetic joint infection) and group two (suspected but non-infected prosthetic joint replacement). Considering patients' final diagnoses, this study aimed to compare the results of the blood culture bottle (BCB) method and the standard sterile fluid culture method.
Results: When cultivated in a blood culture flask, the sensitivity of the culture test was 28.09%, specificity was 95.77%, and accuracy was 58.13%. When cultivated in a solid medium, sensitivity was 10.11%, specificity was 100%, and accuracy was 50%.
Conclusions:When prosthetic joint infection is suspected, BCB usage is a preferable, safer method compared to the standard sterile fluid culture method because it has the power to isolate more bacteria with a higher diagnostic value.