Akademik Veri Yönetim Sistemi
Acta Medica Mediterranea, cilt.30, sa.3, ss.679-683, 2014 (SCI-Expanded)
Introduction: Aims: HPV-DNA testing is widely used throughout the world and today is an important part of cervical cancer screening programs. In this study, it was aimed the investigation of the presence and typing of HPV-DNA by using real time polymerase chain reaction (PCR) in the cervical samples of women with risk factors in terms of HPV and the evaluation of compliance of identified HPV types with results of cervical smear. Methods: HPV-DNA results were analyzed between January 2009 and December 2012 retrospectively. Results: HPV-DNA detection rate was 48% at the cervical samples. The positive samples were described HPV-16 in 26.5%, HPV-18 in 5.5%, non-HPV-16-18 (HPV-31, HPV-35, HPV-59) in 40%, potential HR-HPV (HPV-66) in 1.5% and LR-HPV (HPV-6, HPV-11) in 27.5%. In the cytological examination, %15 (n: 54) samples were evaluated as positive for intraepithelial lesion. These was determined as ASCUS (Atypical Squamous Cells of Undetermined Significance) in 18% (n: 10), LSIL (Low-grade Intraepithelial Lesion) in 50% (n: 27), HSIL (High-grade intraepithelial lesion) in 30% (n: 16), squamous cell carcinoma in 2% samples. In the samples that intraepitelyal lesion was positive but undetermined HPV-DNA, 5 samples were identified as ASCUS and 13 samples were LSIL. Cytological abnormality was found in the 23.4% of 128 detected samples to HR-HPV and potential HR-HPV; in 12.2% of 49 detected samples to LR-HPV and in 9.3% of 194 undetected samples to HPV-DNA. In the samples with cytological abnormality, HPV types were listed HPV-16, non-HPV 16-18, LR-HPV, HPV-18 and potential HR-HPV, respectively, in the samples that cytological abnormality was not found, HPV types were non-HPV-16-18, LR-HPV, HPV-16, HPV-18 and potential HR-HPV. Coclusion: Non-HPV-16-18 were monitored as the most common type and HPV-16 was third of frequency in cytological abnormality negative group; non-HPV-16-18 and HPV-16 were monitored close proportions of each other in cytological abnormality positive group. These isolates were accompany to preinvasive lesions.