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Journal of Enzyme Inhibition and Medicinal Chemistry, vol.27, no.5, pp.680-684, 2012 (SCI-Expanded, Scopus)
Carbonic anhydrase inhibitors (CAIs) are a class of pharmaceuticals used as antiglaucoma agents, diuretics and antiepileptics. Thus, discovery of novel CAIs has become of great importance in the recent years. In the current study, in vitro and in vivo inhibition effects of benzodiazepine drugs, diazepam and midazolam, on human erythrocytes carbonic anhydrase I and II isozymes were investigated. After purification of the isoenzymes, in vitro inhibition assays were performed and Ki values were determined to be of 141.5 μM and 40.7 μM for hCA I and of 5.11 μM and 0.58 μM against hCA II by the esterase activity assay, respectively. The drugs showed strong inhibitory effects on hCA II, in the same range as the clinically used sulphonamide acetazolamide. For in vivo studies, five adult male New Zealand White rabbits (34.2kg) were selected for intravenous administrations of the drugs (2mg/kg and 0.2mg/kg body weight, respectively). The enzyme was significantly inhibited by 2mg/kg diazepam (p<0.05), and 0.2mg/kg midazolam (p<0.05) for up to 30min following intravenous administration. © 2012 Informa UK, Ltd.