Voltammetric and impedimetric detection of DNA hybridization by using dendrimer modified graphite electrodes

Mese F., ÇONGUR G., Erdem A.

Journal of Electroanalytical Chemistry, vol.719, pp.92-97, 2014 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 719
  • Publication Date: 2014
  • Doi Number: 10.1016/j.jelechem.2014.02.007
  • Journal Name: Journal of Electroanalytical Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.92-97
  • Keywords: Cyclic voltammetry, DNA biosensor, Electrochemical impedance spectroscopy, PAMAM dendrimer, Pencil graphite electrode
  • Bilecik Şeyh Edebali University Affiliated: Yes

Abstract

In the present study, the electrochemical sensor was designed by the modification of the fourth generation poly(amido amine) (PAMAM) dendrimer onto the surface of pencil graphite electrode (G4-PDR/PGE) and this single-use dendrimer modified electrode was then examined for advanced nucleic acid detection by using electrochemical techniques. After the covalent immobilization of G4-PDR onto the surface of carboxylated PGE surface, the surface characterization of dendrimer modified PGE was investigated by using scanning electron microscopy (SEM), and also, different electrochemical techniques; cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The voltammetric monitoring of a sequence-selective DNA hybridization related to Hepatitis B virus (HBV) sequences was accordingly performed in the presence of the complementary of probe (target), and also the mixture medium containing target:non-complementary (NC) sequences (1:1). © 2014 Elsevier B.V. All rights reserved.