Effect of α-tocopherol on antioxidant enzyme activities and lipid peroxidation in rainbow trout (Oncorhynchus mykiss)
Acta Veterinaria Hungarica, vol.53, no.4, pp.425-433, 2005 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 53 Issue: 4
- Publication Date: 2005
- Doi Number: 10.1556/avet.53.2005.4.3
- Journal Name: Acta Veterinaria Hungarica
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.425-433
- Keywords: α-Tocopherol, Antioxidant enzyme, Lipid peroxidation, Trout
- Bilecik Şeyh Edebali University Affiliated: Yes
Abstract
In this study, the effect of α-tocopherol on the antioxidant capacity rainbow trout (Oncorhynchus mykiss) was determined. For this purpose, activities of antioxidant enzymes such as catalase (CAT), peroxidase (POD) and glutathione reductase (GSSG-Rx) were investigated. Enzyme activities were measured at 0 (control), 1, 3 and 5 h after α-tocopherol injection. In addition, the levels malondialdehyde (MDA) as a lipid peroxidation marker were determined in the erythrocytes. The results showed that α-tocopherol significantly activated the CAT, POD and GSSG-Rx enzymes as compared with the enzyme activities found in the controls (p < 0.05). However, MDA levels were significantly decreased by α-tocopherol treatment (p < 0.05). The results suggest that α-tocopherol may have a pro-oxidant tendency at a high dose and cause mild oxidative stress which could modulate signal transduction cascades, redirect gene expression, and influence many cellular responses such as proliferation, differentiation, and reproduction. For this reason, α-tocopherol should be used carefully in all applications relation to fish. © 2005 Akadémiai Kiadó, Budapest.