Analysis of fluorescence quenching of pyronin B and pyronin Y by molecular oxygen in aqueous solution
Journal of Luminescence, cilt.126, sa.1, ss.103-108, 2007 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 126 Sayı: 1
- Basım Tarihi: 2007
- Doi Numarası: 10.1016/j.jlumin.2006.05.009
- Dergi Adı: Journal of Luminescence
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.103-108
- Anahtar Kelimeler: Dynamic and static quenching, Molecular oxygen, Pyronin B and pyronin Y
- Bilecik Şeyh Edebali Üniversitesi Adresli: Hayır
Özet
The fluorescence quenching of pyronin B and pyronin Y molecules by molecular oxygen in aqueous solution was studied by using steady-state and time-resolved fluorescence and UV-Vis absorption spectroscopy techniques. In order to understand the quenching mechanism, fluorescence decays, absorption and fluorescence spectra of the probes were recorded as a function of the oxygen concentration and temperature. The quenching was found to be appreciable and shows positive deviation in the Stern-Volmer representation obtained from the fluorescence intensity ratio. Fluorescence quenching constants (kq) were calculated from the τo/τ vs. [Q] plots having linear correlation and compared with calculated diffusion-controlled rate constants (kdiff) values. Experimental results were in good agreement with the simultaneous dynamic and static quenching model. © 2006 Elsevier B.V. All rights reserved.