Analysis of fluorescence quenching of pyronin B and pyronin Y by molecular oxygen in aqueous solution

ÇELEBİ N., ARK M., ONGANER Y.

Journal of Luminescence, vol.126, no.1, pp.103-108, 2007 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 126 Issue: 1
  • Publication Date: 2007
  • Doi Number: 10.1016/j.jlumin.2006.05.009
  • Journal Name: Journal of Luminescence
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.103-108
  • Keywords: Dynamic and static quenching, Molecular oxygen, Pyronin B and pyronin Y
  • Bilecik Şeyh Edebali University Affiliated: No

Abstract

The fluorescence quenching of pyronin B and pyronin Y molecules by molecular oxygen in aqueous solution was studied by using steady-state and time-resolved fluorescence and UV-Vis absorption spectroscopy techniques. In order to understand the quenching mechanism, fluorescence decays, absorption and fluorescence spectra of the probes were recorded as a function of the oxygen concentration and temperature. The quenching was found to be appreciable and shows positive deviation in the Stern-Volmer representation obtained from the fluorescence intensity ratio. Fluorescence quenching constants (kq) were calculated from the τo/τ vs. [Q] plots having linear correlation and compared with calculated diffusion-controlled rate constants (kdiff) values. Experimental results were in good agreement with the simultaneous dynamic and static quenching model. © 2006 Elsevier B.V. All rights reserved.