Research Information System
Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi, vol.6, no.1a, pp.204-212, 2019 (Peer-Reviewed Journal)
Sphingolipids play critical role in biological processes such cell death, survival and drug resistancein many cancers. Ceramide and dihydroceramide are proliferation and death associated sphingolipids.Recent cancer research are focused on clarifying cancer-sphingolipid metabolism. In last years, ceramide asa key molecule in sphingolipid metabolism relationship has been investigated for its anticancer activity viaaugmenting its intracellular level by ceramidase inhibitor application. Prostate cancer is among the most frequenthuman cancers and is reported as second most common cancer-related death cause. Prostate cancer is common atages older than 65. The aim of this study was to investigate the potential cytotoxic activity of a ceramidase inhibitor(1S,2R)-D-erythro-2-(N-Myristoylamino)-1-phenyl-1-propanol (D-erythro-MAPP) on human prostate cancer DU-145cells by using MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay, flow cytometry, confocaland TEM microscopy. MTT findings showed that D-erythro-MAPP caused toxicity in low doses on DU-145 cells.Confocal and TEM microscopy findings indicated hole formation in cytoskeleton, chromatin condensation, horseshoeshaped cell nuclei as morphological changes and blebbings on cell membrane, fragmentation of nuclei, chromatincondensation and loss of cristae as ultrastructural changes, respectively. Flow cytometry findings showed that Derythro-MAPP triggered apoptosis in short-term application of 24 hours on DU-145 cells. According to results it canbe concluded that D-erythro-MAPP decreased viability of DU-145 cells in dose-dependent manner. Our findingsstated the anti-cancer and cytotoxic potential of D-erythro-MAPP on DU-145 and this agent might be used in drugdeveloping for cancer treatment after the further in vitro and in vivo studies.