Saxitoxin aptasensor based on attenuated internal reflection ellipsometry for seafood

ÇAĞLAYAN M. O., Üstündağ Z.

Toxicon, cilt.187, ss.255-261, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 187
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.toxicon.2020.09.005
  • Dergi Adı: Toxicon
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, EMBASE, Environment Index, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.255-261
  • Anahtar Kelimeler: Attenuated internal reflection ellipsometry, Paralytic shellfish poisoning toxin, Saxitoxin, Seafood, Surface plasmon resonance
  • Bilecik Şeyh Edebali Üniversitesi Adresli: Evet

Özet

© 2020 Elsevier LtdIn this study, we proposed label-free saxitoxin (STX) sensor using STX specific aptamer in combination with spectroscopic ellipsometry (SE) and attenuated internal reflection (AIR) spectroscopic ellipsometry method which is operated under surface plasmon resonance (SPR) conditions. Besides the other surface plasmon resonance-based applications, AIR-SE applications have unique advantages in terms of sensitivity and it was used herein for real-time detection of STX in real samples. Another method, SE, was also used and compared with AIR-SE. Analytical performances were satisfactory with low detection limits and a wide detection range. Limit of detection was 0.01 ng/mL for AIR-SE and 0.11 ng/mL for SE. Both proposed sensors were operable in 0.01 nM–1000 nM STX range. These methods were also used for the accurate, selective, and sensitive detection of STX from fish and shrimp samples.