Mercuric ion detection by plasmon-enhanced spectrophotometric ellipsometer using specific oligonucleotide probes

ÇAĞLAYAN M. O.

Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, cilt.241, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 241
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.saa.2020.118682
  • Dergi Adı: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chimica, Compendex, EMBASE, INSPEC, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Biosensor, Mercuric ion, Mercury specific oligonucleotide probes, Spectrophotometric ellipsometry, Surface plasmon resonance, Total internal reflection
  • Bilecik Şeyh Edebali Üniversitesi Adresli: Evet

Özet

© 2020 Elsevier B.V.Pollution due to heavy metal ions, including mercury, has become a major issue because of their toxicities. It is required to monitor mercury levels in aqueous media using fast and selective methods with high accuracy. Ellipsometry is a promising technique for instance when it's combined with the plasmon resonance phenomena. We reported a biosensor system available for qualitative/quantitative determination of mercuric ions in aqueous media where both the spectrophotometric ellipsometry and oligonucleotide recognition elements were used. A single step assay using both a linear (ProbeL) and a hair-pin (ProbeH) type oligonucleotide probe as a recognition element, in addition to a sandwich-type (ProbeLS) assay were developed and compared. The detection limits were 0.23 nM, 0.03 nM and 0.15 pM for ProbeL, ProbeH and ProbeLS, respectively. The detection range was between 0.05 nM and 100 nM Hg2+ for all assays proposed herein.