Phytase from Weissella halotolerans: purification, partial characterisation and the effect of some metals
International Journal of Food Properties, vol.20, pp.2127-2137, 2017 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 20
- Publication Date: 2017
- Doi Number: 10.1080/10942912.2017.1368547
- Journal Name: International Journal of Food Properties
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.2127-2137
- Keywords: Characterisation, Enzyme purification, Metal ions, Phytase, Weissella halotolerans
- Bilecik Şeyh Edebali University Affiliated: Yes
Abstract
In the study, phytase was purified in three simple steps comprising ammonium sulphate precipitation, anion exchange and gel filtration chromatography from Weissella halotolerans. The enzyme was obtained with a specific activity of 227.73 EU/mg and 6.52% recovery. The molecular mass of the enzyme was determined to be 41.52 kDa. The optimum pH and temperature for the enzyme were 6.0 and 50°C, respectively. Furthermore, the effects of metal ions on the enzyme were investigated. Ag+, Zn2+, Cr2+ and Fe2+ ions inhibited phytase by 21.86, 25.63, 32.82 and 90.43%, respectively, whereas Co2+, Cu2+, Pb2+, Cd2+ ve Mn2+ ions increased the enzyme activity.