Phytase from Weissella halotolerans: purification, partial characterisation and the effect of some metals

Demir Y., ŞENOL KOTAN M., DİKBAŞ N., BEYDEMİR Ş.

International Journal of Food Properties, cilt.20, ss.2127-2137, 2017 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1080/10942912.2017.1368547
  • Dergi Adı: International Journal of Food Properties
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2127-2137
  • Anahtar Kelimeler: Characterisation, Enzyme purification, Metal ions, Phytase, Weissella halotolerans
  • Bilecik Şeyh Edebali Üniversitesi Adresli: Evet

Özet

In the study, phytase was purified in three simple steps comprising ammonium sulphate precipitation, anion exchange and gel filtration chromatography from Weissella halotolerans. The enzyme was obtained with a specific activity of 227.73 EU/mg and 6.52% recovery. The molecular mass of the enzyme was determined to be 41.52 kDa. The optimum pH and temperature for the enzyme were 6.0 and 50°C, respectively. Furthermore, the effects of metal ions on the enzyme were investigated. Ag+, Zn2+, Cr2+ and Fe2+ ions inhibited phytase by 21.86, 25.63, 32.82 and 90.43%, respectively, whereas Co2+, Cu2+, Pb2+, Cd2+ ve Mn2+ ions increased the enzyme activity.