Apoptotic, antioxidant and antiradical effects of majdine and isomajdine from Vinca herbacea Waldst. and kit

GÜLÇİN İ., BEYDEMİR Ş., Topal F., Gagua N., Bakuridze A., Bayram R., ...More

Journal of Enzyme Inhibition and Medicinal Chemistry, vol.27, no.4, pp.587-594, 2012 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 4
  • Publication Date: 2012
  • Doi Number: 10.3109/14756366.2011.604318
  • Journal Name: Journal of Enzyme Inhibition and Medicinal Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.587-594
  • Keywords: Antioxidant activity, Apoptotic effect, Isomajdine, Majdine, Radical scavenging
  • Bilecik Şeyh Edebali University Affiliated: Yes

Abstract

In the present study, apoptotic, antioxidant and antiradical effects of majdine and isomajdine from Vinca herbacea Waldst. and Kit were studied. For testing the possible apoptotic effects of majdine and isomajdine from V. herbacea, DNA fragmentation assay was conducted on the rat brain cortical tissue homogenates, in vitro. Also their possible effects on mitochondrial activity were tested by using the same tissue samples of rats. In addition, the antioxidant activity of isomajdine and majdine was determined using various in vitro antioxidant assays, including 2,2′-azino-bis(3-ethylbenzthiazoline- 6-sulfonic acid) (ABTS•+) radical scavenging and N,N-dimethyl-p-phenylenediamine (DMPD•+) radical scavenging, ferric ions (Fe3+) and cupric ions (Cu2+) reducing abilities and ferrous ions (Fe2+) chelating activity. On the other hand, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), α-tocopherol and trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) were used as reference antioxidants. © 2012 Informa UK, Ltd.