Hydroxyapatite Nanoparticles Modified Graphite Electrodes for Electrochemical DNA Detection

Erdem A., Çongur G.

Electroanalysis, cilt.30, sa.1, ss.67-74, 2018 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Sayı: 1
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1002/elan.201700462
  • Dergi Adı: Electroanalysis
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.67-74
  • Anahtar Kelimeler: cyclic voltammetry (CV), differential pulse voltammetry (DPV), electrochemical biosensor, electrochemical impedance spectroscopy (EIS), Hydroxyapatite nanoparticles (HaNPs), pencil graphite electrode
  • Bilecik Şeyh Edebali Üniversitesi Adresli: Evet

Özet

Hydroxyapatite nanoparticles (HaNP) modified pencil graphite electrodes (PGEs) were developed for the first time in the literature, and accordingly they were applied for electrochemical monitoring of sequence-selective DNA hybridization. The experimental conditions for HaNP modification of PGE, and DNA hybridization related to Hepatitis B Virus (HBV) DNA sequence were optimized. The microscopic and electrochemical characterization of HaNP-PGE in contrast to the unmodified one was utilized. Under optimized experimental conditions, the selectivity of HBV DNA probe immobilized biosensor was tested against to non-complementary (NC), mismatch (MM) sequences and the mixture of target:NC (1 : 1) or target: MM (1 : 1).