Succinamic acid functionalized PAMAM dendrimer modified pencil graphite electrodes for voltammetric and impedimetric DNA analysis

ÇONGUR G., Eksin E., Mese F., Erdem A.

Sensors and Actuators, B: Chemical, vol.201, pp.59-64, 2014 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 201
  • Publication Date: 2014
  • Doi Number: 10.1016/j.snb.2014.03.104
  • Journal Name: Sensors and Actuators, B: Chemical
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.59-64
  • Keywords: Cyclic voltammetry, Differential pulse voltammetry, Electrochemical DNA biosensor, Electrochemical impedance spectroscopy, Pencil graphite electrode, Succinamic acid functionalized second generation PAMAM dendrimer
  • Bilecik Şeyh Edebali University Affiliated: Yes

Abstract

In the present study, the surface of pencil graphite electrode (PGE) was modified using succinamic acid functionalized second generation poly (amidoamine) (PAMAM) dendrimer (G2-PS) to design a novel single-use electrochemical DNA biosensor. The microscopic characterization of G2-PS/PGEs was performed by using scanning electron microscopy (SEM). The effects of the experimental parameters, such as dendrimer concentration, activation of PGE surface, DNA concentration, and different labelling of DNA using chemical groups were explored upon to the electrode response by using different electrochemical techniques; differential pulse voltammetry (DPV), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). After immobilization of calf thymus double stranded DNA (ctDNA), or short DNA oligonucleotide (DNA ODN) onto the surface of dendrimer modified electrode, an enhanced sensor response was recorded using G2-PS/PGEs in contrast to one of unmodified PGEs. The features of dendrimer modified DNA biosensor were comprehensively discussed in comparison to earlier reports. © 2014 Elsevier B.V. All rights reserved.