Impedimetric Detection of microRNA at Graphene Oxide Modified Sensors

Çongur G., Eksin E., Erdem A.

Electrochimica Acta, vol.172, pp.20-27, 2015 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 172
  • Publication Date: 2015
  • Doi Number: 10.1016/j.electacta.2015.03.210
  • Journal Name: Electrochimica Acta
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.20-27
  • Keywords: electrochemical impedance spectroscopy, fetal bovine serum, Graphene oxide, microRNA, pencil graphite electrode
  • Bilecik Şeyh Edebali University Affiliated: Yes

Abstract

Abstract The graphene oxide (GO) modified pencil graphite electrodes (PGEs) were utilized for electrochemical monitoring of microRNA-34a (miRNA-34a) in this study. The GO concentration was firstly optimized after its modification onto the surface of disposable PGE by passive adsorption. The electrochemical behaviour of GO-PGEs was investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) as well as microscopic characterization by using scanning electron microscopy (SEM). The impedimetric detection of hybridization between miRNA-34a target and its complementary DNA probe was recognized under the optimum conditions. The selectivity of the impedimetric genosensor was furtherly studied against to other miRNA sequences; such as; miRNA-15a, miRNA-155 and miRNA-660 in PBS (pH 7.40) or fetal bovine serum (FBS): PBS (1:1) diluted solution.