An ellipsometric biosensor using aptamer for the detection of mercuric ions

Ertan T., ÇAĞLAYAN M. O.

Chemical Papers, cilt.75, sa.1, ss.89-97, 2021 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 75 Sayı: 1
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1007/s11696-020-01282-3
  • Dergi Adı: Chemical Papers
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core
  • Sayfa Sayıları: ss.89-97
  • Anahtar Kelimeler: Biosensor, DNA aptamer, Ellipsometry, Mercuric ion
  • Bilecik Şeyh Edebali Üniversitesi Adresli: Evet

Özet

© 2020, Institute of Chemistry, Slovak Academy of Sciences.An ellipsometric biosensor, to be used in the detection of mercuric ions that affect the vital organs of living organisms, was developed. In this context, as a diagnostic element of the developed ellipsometric biosensor, a nucleic acid-based aptamer that is highly selective for mercuric ions (Hg2+) was used. Two different aptamers, a long and straight sequence, and a hairpin-structured oligonucleotide sequence were chosen. The detection sensitivity obtained by both aptamers was 0.237°/μM and 0.211°/μM, respectively. The detection limit was 0.89 pM Hg2+and 187 nM Hg2+ for linear- and hairpin-type oligonucleotide, respectively.