An ellipsometric biosensor using aptamer for the detection of mercuric ions
Chemical Papers, vol.75, no.1, pp.89-97, 2021 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 75 Issue: 1
- Publication Date: 2021
- Doi Number: 10.1007/s11696-020-01282-3
- Journal Name: Chemical Papers
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core
- Page Numbers: pp.89-97
- Keywords: Biosensor, DNA aptamer, Ellipsometry, Mercuric ion
- Bilecik Şeyh Edebali University Affiliated: Yes
Abstract
© 2020, Institute of Chemistry, Slovak Academy of Sciences.An ellipsometric biosensor, to be used in the detection of mercuric ions that affect the vital organs of living organisms, was developed. In this context, as a diagnostic element of the developed ellipsometric biosensor, a nucleic acid-based aptamer that is highly selective for mercuric ions (Hg2+) was used. Two different aptamers, a long and straight sequence, and a hairpin-structured oligonucleotide sequence were chosen. The detection sensitivity obtained by both aptamers was 0.237°/μM and 0.211°/μM, respectively. The detection limit was 0.89 pM Hg2+and 187 nM Hg2+ for linear- and hairpin-type oligonucleotide, respectively.