Impedimetric detection of in situ interaction between anti-cancer drug bleomycin and DNA

Erdem A., ÇONGUR G.

International Journal of Biological Macromolecules, vol.61, pp.295-301, 2013 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 61
  • Publication Date: 2013
  • Doi Number: 10.1016/j.ijbiomac.2013.07.012
  • Journal Name: International Journal of Biological Macromolecules
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.295-301
  • Keywords: Bleomycin, Cis-platin, Drug-DNA interactions, Electrochemical impedance spectroscopy, Mitomycin C
  • Bilecik Şeyh Edebali University Affiliated: Yes

Abstract

Surface confined interaction of anti-cancer drug bleomycin (BLM) with nucleic acids: single stranded and double stranded DNA was investigated herein by using electrochemical impedance spectroscopy (EIS) technique in combination with a graphite sensor technology. The experimental conditions were optimized: such as, dsDNA concentration, BLM concentration and interaction time. The main features of impedimetric DNA biosensor, such as its detection limit and the repeatability, were also discussed. The in situ interaction of BLM with dsDNA was also tested impedimetrically in the absence or presence of other chemotherapeutic agents, such as mitomycin C (MC) and cis-platin ( cis-DDP) for testing the selectivity. © 2013 Elsevier B.V.